EC number:2.4.1.85
| ID: | 2.4.1.85 |
|---|---|
| Description: | Cyanohydrin beta-glucosyltransferase. |
| Alternative Name: |
Uridine diphosphoglucose-p-hydroxymandelonitrile glucosyltransferase. Uridine diphosphoglucose-cyanohydrin glucosyltransferase. UGT85B1. UDP-glucose-p-hydroxymandelonitrile glucosyltransferase. |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 2.4.1.85 |
| BRENDA Enzyme Link: | BRENDA 2.4.1.85 |
| KEGG Enzyme Link: | KEGG2.4.1.85 |
| BioCyc Enzyme Link: | BioCyc 2.4.1.85 |
| ExPASy Enzyme Link: | ExPASy2.4.1.85 |
| EC2PDB Enzyme Link: | EC2PDB 2.4.1.85 |
| ExplorEnz Enzyme Link: | ExplorEnz 2.4.1.85 |
| PRIAM enzyme-specific profiles Link: | PRIAM 2.4.1.85 |
| IntEnz Enzyme Link: | IntEnz 2.4.1.85 |
| MEDLINE Enzyme Link: | MEDLINE 2.4.1.85 |
| RHEA:12853 | (S)-4-hydroxymandelonitrile + UDP-alpha-D-glucose = dhurrin + H(+) + UDP |
| RULE(radius=1) | [*:1]-[O;H0;+0:2]-[CH;+0:3](-[*:4])-[*:5].[*:6]-[OH;+0:7]>>[*:4]-[CH;+0:3](-[*:5])-[O;H0;+0:7]-[*:6].[*:1]-[OH;+0:2] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Determination of catalytic key amino acids and UDP sugar donor specificity of the cyanohydrin glycosyltransferase UGT85B1 from Sorghum bicolor. Molecular modeling substantiated by site-specific mutagenesis and biochemical analyses. | Thorsøe KS, Bak S, Olsen CE, Imberty A, Breton C, Lindberg Møller B | 2005 Oct | 16169969 |
| Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome. | Kristensen C, Morant M, Olsen CE, Ekstrøm CT, Galbraith DW, Møller BL, Bak S | 2005 Feb 1 | 15665094 |
| The in vitro substrate regiospecificity of recombinant UGT85B1, the cyanohydrin glucosyltransferase from Sorghum bicolor. | Hansen KS, Kristensen C, Tattersall DB, Jones PR, Olsen CE, Bak S, Møller BL | 2003 Sep | 12946413 |
| Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants. | Busk PK, Møller BL | 2002 Jul | 12114576 |
| The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. Isolation, cloning, heterologous expression, and substrate specificity. | Jones PR, Moller BL, Hoj PB | 1999 Dec 10 | 10585420 |