EC number:2.4.99.1
Enzyme
Download| EC Tree |
| 2. Transferases |
| 2.4 Glycosyltransferases |
| 2.4.99 Transferring other glycosyl groups |
| ID: | 2.4.99.1 |
|---|---|
| Description: | Beta-galactoside alpha-(2,6)-sialyltransferase. |
| Alternative Name: |
Lactosylceramide-alpha-2,6-N-sialyltransferase. CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase. Beta-galactoside alpha-2,6-sialyltransferase. Beta-galactosamide alpha-2,6-sialyltransferase. |
| Cath: | 3.90.1480.20; |
3D structure
Click one PDB to see exact 3D structure provided by NGL.
Note: Use your mouse to drag, rotate, and zoom in and out of the structure. Mouse-over to identify atoms and bonds. Mouse controls documentation.References
External Links
| UniProtKB Enzyme Link: | UniProtKB 2.4.99.1 |
| BRENDA Enzyme Link: | BRENDA 2.4.99.1 |
| KEGG Enzyme Link: | KEGG2.4.99.1 |
| BioCyc Enzyme Link: | BioCyc 2.4.99.1 |
| ExPASy Enzyme Link: | ExPASy2.4.99.1 |
| EC2PDB Enzyme Link: | EC2PDB 2.4.99.1 |
| ExplorEnz Enzyme Link: | ExplorEnz 2.4.99.1 |
| PRIAM enzyme-specific profiles Link: | PRIAM 2.4.99.1 |
| IntEnz Enzyme Link: | IntEnz 2.4.99.1 |
| MEDLINE Enzyme Link: | MEDLINE 2.4.99.1 |
| RHEA:52104 | a beta-D-galactoside + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl derivative + CMP + H(+) |
| RULE(radius=1) | [*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[O;H0;+0:5]-[*:6].[*:7]-[OH;+0:8]>>[*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[O;H0;+0:8]-[*:7].[*:6]-[OH;+0:5] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose. | Paulson JC, Beranek WE, Hill RL | 1977 Apr 10 | 849932 |
| Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid sialyltransferase. | Spiro MJ, Spiro RG | 1968 Dec 25 | 5726897 |
| The sialic acids. XV. Transfer of sialic acid to glycoproteins by a sialyltransferase from colostrum. | Bartholomew BA, Jourdian GW, Roseman S | 1973 Aug 25 | 4723915 |
| Physical and chemical studies on ceruloplasmin. 8. Preparation of N-acetylneuraminic acid-1-14C-labeled ceruloplasmin. | Hickman J, Ashwell G, Morell AG, van den Hamer CJ, Scheinberg IH | 1970 Feb 25 | 4313609 |
| Enzymatic properties of beta-D-galactoside alpha2 leads to 6 sialytransferase from bovine colostrum. | Paulson JC, Rearick JI, Hill RL | 1977 Apr 10 | 849933 |
| The sialyltransferase "sialylmotif" participates in binding the donor substrate CMP-NeuAc. | Datta AK, Paulson JC | 1995 Jan 27 | 7829476 |
| Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type. | Schachter H, Narasimhan S, Gleeson P, Vella G | 1983 | 6366476 |
| Primary structure of beta-galactoside alpha 2,6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor. | Weinstein J, Lee EU, McEntee K, Lai PH, Paulson JC | 1987 Dec 25 | 3121604 |
| The structure of human α-2,6-sialyltransferase reveals the binding mode of complex glycans. | Kuhn B, Benz J, Greif M, Engel AM, Sobek H, Rudolph MG | 2013 Sep | 23999306 |
| Universal phosphatase-coupled glycosyltransferase assay. | Wu ZL, Ethen CM, Prather B, Machacek M, Jiang W | 2011 Jun | 21081508 |
| Probing the substrate specificity of four different sialyltransferases using synthetic beta-D-Galp-(1-->4)-beta-D-GlcpNAc-(1-->2)-alpha-D-Manp-(1-->O) (CH(2))7CH3 analogues general activating effect of replacing N-acetylglucosamine by N-propionylglucosamine. | Rohfritsch PF, Joosten JA, Krzewinski-Recchi MA, Harduin-Lepers A, Laporte B, Juliant S, Cerutti M, Delannoy P, Vliegenthart JF, Kamerling JP | 2006 Apr | 16439063 |
| Comparison of the enzymatic properties of mouse beta-galactoside alpha2,6-sialyltransferases, ST6Gal I and II. | Takashima S, Tsuji S, Tsujimoto M | 2003 Aug | 12966079 |
| Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II. | Krzewinski-Recchi MA, Julien S, Juliant S, Teintenier-Lelièvre M, Samyn-Petit B, Montiel MD, Mir AM, Cerutti M, Harduin-Lepers A, Delannoy P | 2003 Mar | 12603328 |
| Characterization of the second type of human beta-galactoside alpha 2,6-sialyltransferase (ST6Gal II), which sialylates Galbeta 1,4GlcNAc structures on oligosaccharides preferentially. Genomic analysis of human sialyltransferase genes. | Takashima S, Tsuji S, Tsujimoto M | 2002 Nov 29 | 12235148 |
| Purification and characterization of an endogenous inhibitor of the sialyltransferase CMP-N-acetylneuraminate: lactosylceramide alpha 2,6-N-acetylneuraminyltransferase (EC 2.4.99.-). | Albarracin I, Lassaga FE, Caputto R | 1988 Sep 1 | 2460092 |
| RHEA:21552 | beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = CMP + H(+) + N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide |
| RULE(radius=1) | [*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[O;H0;+0:5]-[*:6].[*:7]-[OH;+0:8]>>[*:7]-[O;H0;+0:8]-[C;H0;+0:2](-[*:1])(-[*:3])-[*:4].[*:6]-[OH;+0:5] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and characterization of an endogenous inhibitor of the sialyltransferase CMP-N-acetylneuraminate: lactosylceramide alpha 2,6-N-acetylneuraminyltransferase (EC 2.4.99.-). | Albarracin I, Lassaga FE, Caputto R | 1988 Sep 1 | 2460092 |
| RHEA:11836 | CMP-N-acetyl-beta-neuraminate + N-acetyllactosamine = CMP + H(+) + N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine |
| RULE(radius=1) | [*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[O;H0;+0:5]-[*:6].[*:7]-[OH;+0:8]>>[*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[O;H0;+0:8]-[*:7].[*:6]-[OH;+0:5] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Enzymatic properties of beta-D-galactoside alpha2 leads to 6 sialytransferase from bovine colostrum. | Paulson JC, Rearick JI, Hill RL | 1977 Apr 10 | 849933 |
| The sialyltransferase "sialylmotif" participates in binding the donor substrate CMP-NeuAc. | Datta AK, Paulson JC | 1995 Jan 27 | 7829476 |
| Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type. | Schachter H, Narasimhan S, Gleeson P, Vella G | 1983 | 6366476 |
| Primary structure of beta-galactoside alpha 2,6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor. | Weinstein J, Lee EU, McEntee K, Lai PH, Paulson JC | 1987 Dec 25 | 3121604 |
| The structure of human α-2,6-sialyltransferase reveals the binding mode of complex glycans. | Kuhn B, Benz J, Greif M, Engel AM, Sobek H, Rudolph MG | 2013 Sep | 23999306 |
| Universal phosphatase-coupled glycosyltransferase assay. | Wu ZL, Ethen CM, Prather B, Machacek M, Jiang W | 2011 Jun | 21081508 |
| Probing the substrate specificity of four different sialyltransferases using synthetic beta-D-Galp-(1--&gt;4)-beta-D-GlcpNAc-(1--&gt;2)-alpha-D-Manp-(1--&gt;O) (CH(2))7CH3 analogues general activating effect of replacing N-acetylglucosamine by N-propionylglucosamine. | Rohfritsch PF, Joosten JA, Krzewinski-Recchi MA, Harduin-Lepers A, Laporte B, Juliant S, Cerutti M, Delannoy P, Vliegenthart JF, Kamerling JP | 2006 Apr | 16439063 |
| Comparison of the enzymatic properties of mouse beta-galactoside alpha2,6-sialyltransferases, ST6Gal I and II. | Takashima S, Tsuji S, Tsujimoto M | 2003 Aug | 12966079 |
| Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II. | Krzewinski-Recchi MA, Julien S, Juliant S, Teintenier-Lelièvre M, Samyn-Petit B, Montiel MD, Mir AM, Cerutti M, Harduin-Lepers A, Delannoy P | 2003 Mar | 12603328 |
| Characterization of the second type of human beta-galactoside alpha 2,6-sialyltransferase (ST6Gal II), which sialylates Galbeta 1,4GlcNAc structures on oligosaccharides preferentially. Genomic analysis of human sialyltransferase genes. | Takashima S, Tsuji S, Tsujimoto M | 2002 Nov 29 | 12235148 |