Enzyme

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     2. Transferases
        2.4 Glycosyltransferases
            2.4.99 Transferring other glycosyl groups
ID:2.4.99.12
Description:Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase.
Alternative Name: Lipid IV(A) KDO transferase.
KDO transferase.
3-deoxy-manno-octulosonic acid transferase.
3-deoxy-D-manno-oct-2-ulosonic acid transferase.
Cath: 3.40.50.11720; 3.40.50.20; 3.40.50.200; 3.40.50.2000;

3D structure

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References

External Links

UniProtKB Enzyme Link: UniProtKB 2.4.99.12
BRENDA Enzyme Link: BRENDA 2.4.99.12
KEGG Enzyme Link: KEGG2.4.99.12
BioCyc Enzyme Link: BioCyc 2.4.99.12
ExPASy Enzyme Link: ExPASy2.4.99.12
EC2PDB Enzyme Link: EC2PDB 2.4.99.12
ExplorEnz Enzyme Link: ExplorEnz 2.4.99.12
PRIAM enzyme-specific profiles Link: PRIAM 2.4.99.12
IntEnz Enzyme Link: IntEnz 2.4.99.12
MEDLINE Enzyme Link: MEDLINE 2.4.99.12
MSA:

2.4.99.12;

Phylogenetic Tree:

2.4.99.12;

Uniprot:
M-CSA:
RHEA:28066 CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) = alpha-Kdo-(2->6)-lipid IVA + CMP + H(+)
RULE(radius=1) [*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[O;H0;+0:5]-[*:6].[*:7]-[OH;+0:8]>>[*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[O;H0;+0:8]-[*:7].[*:6]-[OH;+0:5]
Reaction
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References

TitleAuthorsDatePubMed ID
A mono-functional 3-deoxy-D-manno-octulosonic acid (Kdo) transferase and a Kdo kinase in extracts of Haemophilus influenzae.White KA, Kaltashov IA, Cotter RJ, Raetz CR1997 Jun 279195966
Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-alpha-D-manno-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183.Löbau S, Mamat U, Brabetz W, Brade H1995 Nov8748024
The structures of oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant Escherichia coli strain expressing the gene gseA [3-deoxy-D-manno-octulopyranosonic acid (Kdo) transferase] of Chlamydia psittaci 6BC.Holst O, Bock K, Brade L, Brade H1995 Apr 17744029
Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis.Schmidt H, Hansen G, Singh S, Hanuszkiewicz A, Lindner B, Fukase K, Woodard RW, Holst O, Hilgenfeld R, Mamat U, Mesters JR2012 Apr 1722474366
WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis.Mamat U, Schmidt H, Munoz E, Lindner B, Fukase K, Hanuszkiewicz A, Wu J, Meredith TC, Woodard RW, Hilgenfeld R, Mesters JR, Holst O2009 Aug 1419546212
Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli.Belunis CJ, Raetz CR1992 May 151577828
A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specific epitope.Belunis CJ, Mdluli KE, Raetz CR, Nano FE1992 Sep 151382060
3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase (WaaA) and kdo kinase (KdkA) of Haemophilus influenzae are both required to complement a waaA knockout mutation of Escherichia coli.Brabetz W, Müller-Loennies S, Brade H2000 Nov 1010952982
Comparative analyses of secondary gene products of 3-deoxy-D-manno-oct-2-ulosonic acid transferases from Chlamydiaceae in Escherichia coli K-12.Brabetz W, Lindner B, Brade H2000 Sep10951204
Bordetella pertussis waaA encodes a monofunctional 2-keto-3-deoxy-D-manno-octulosonic acid transferase that can complement an Escherichia coli waaA mutation.Isobe T, White KA, Allen AG, Peacock M, Raetz CR, Maskell DJ1999 Apr10198035