EC number:2.5.1.3
Enzyme
Download| EC Tree |
| 2. Transferases |
| 2.5 Transferring alkyl or aryl groups, other than methyl groups |
| 2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date) |
| ID: | 2.5.1.3 |
|---|---|
| Description: | Thiamine phosphate synthase. |
| Alternative Name: |
TMP pyrophosphorylase. TMP diphosphorylase. Thiamine-phosphate synthase. Thiamine-phosphate pyrophosphorylase. Thiamine-phosphate diphosphorylase. |
| Cath: | 3.20.20.70; 3.40.225.10; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 2.5.1.3 |
| BRENDA Enzyme Link: | BRENDA 2.5.1.3 |
| KEGG Enzyme Link: | KEGG2.5.1.3 |
| BioCyc Enzyme Link: | BioCyc 2.5.1.3 |
| ExPASy Enzyme Link: | ExPASy2.5.1.3 |
| EC2PDB Enzyme Link: | EC2PDB 2.5.1.3 |
| ExplorEnz Enzyme Link: | ExplorEnz 2.5.1.3 |
| PRIAM enzyme-specific profiles Link: | PRIAM 2.5.1.3 |
| IntEnz Enzyme Link: | IntEnz 2.5.1.3 |
| MEDLINE Enzyme Link: | MEDLINE 2.5.1.3 |
| RHEA:47848 | 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[O;H0;+0:3]-[*:4].[*:5]=[C;H0;+0:6](-[OH;+0:7])-[c;H0;+0:8](:[*:9]):[n;H0;+0:10]:[*:11].[H+;H0:12].[H+;H0:13]>>[*:1]-[CH2;+0:2]-[n+;H0:10](:[*:11]):[cH;+0:8]:[*:9].[*:4]-[OH;+0:3].[*:5]=[C;H0;+0:6]=[O;H0;+0:7] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| A Brassica cDNA clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis. | Kim YS, Nosaka K, Downs DM, Kwak JM, Park D, Chung IK, Nam HG | 1998 Aug | 9700068 |
| Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis. | Zhang Y, Taylor SV, Chiu HJ, Begley TP | 1997 May | 9139923 |
| Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway. | Kawasaki Y | 1993 Aug | 8394314 |
| Identification of inhibitors against Mycobacterium tuberculosis thiamin phosphate synthase, an important target for the development of anti-TB drugs. | Khare G, Kar R, Tyagi AK | 2011 | 21818324 |
| Determination of the genetic, molecular, and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1. | Ajjawi I, Tsegaye Y, Shintani D | 2007 Mar 1 | 17174261 |
| A th-1 Mutant of Arabidopsis thaliana Is Defective for a Thiamin-Phosphate-Synthesizing Enzyme: Thiamin Phosphate Pyrophosphorylase. | Komeda Y, Tanaka M, Nishimune T | 1988 Oct | 16666289 |
| The enzymatic synthesis of thiamine monophosphate. | LEDER IG | 1961 Nov | 14463407 |
| The biosynthesis of thiamine. 2. Fractionation of enzyme system and identification of thiazole monophosphate and thiamine monophosphate as intermediates. | CAMIENER GW, BROWN GM | 1960 Aug | 13807175 |
| Systematic discovery of analogous enzymes in thiamin biosynthesis. | Morett E, Korbel JO, Rajan E, Saab-Rincon G, Olvera L, Olvera M, Schmidt S, Snel B, Bork P | 2003 Jul | 12794638 |
| Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution. | Chiu HJ, Reddick JJ, Begley TP, Ealick SE | 1999 May 18 | 10350464 |
| RHEA:47844 | 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[O;H0;+0:3]-[*:4].[*:5]=[C;H0;+0:6](-[OH;+0:7])-[CH;+0:8]1-[N;H0;+0:9]=[C;H0;+0:10](-[*:11])-[C;H0;+0:12](=[CH;+0:13]-[*:14])-[S;H0;+0:15]-1.[H+;H0:16].[H+;H0:17]>>[*:1]-[CH2;+0:2]-[n+;H0:9]1:[cH;+0:8]:[s;H0;+0:15]:[c;H0;+0:12](-[CH2;+0:13]-[*:14]):[c;H0;+0:10]:1-[*:11].[*:4]-[OH;+0:3].[*:5]=[C;H0;+0:6]=[O;H0;+0:7] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| A Brassica cDNA clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis. | Kim YS, Nosaka K, Downs DM, Kwak JM, Park D, Chung IK, Nam HG | 1998 Aug | 9700068 |
| Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis. | Zhang Y, Taylor SV, Chiu HJ, Begley TP | 1997 May | 9139923 |
| Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway. | Kawasaki Y | 1993 Aug | 8394314 |
| Identification of inhibitors against Mycobacterium tuberculosis thiamin phosphate synthase, an important target for the development of anti-TB drugs. | Khare G, Kar R, Tyagi AK | 2011 | 21818324 |
| Determination of the genetic, molecular, and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1. | Ajjawi I, Tsegaye Y, Shintani D | 2007 Mar 1 | 17174261 |
| A th-1 Mutant of Arabidopsis thaliana Is Defective for a Thiamin-Phosphate-Synthesizing Enzyme: Thiamin Phosphate Pyrophosphorylase. | Komeda Y, Tanaka M, Nishimune T | 1988 Oct | 16666289 |
| The enzymatic synthesis of thiamine monophosphate. | LEDER IG | 1961 Nov | 14463407 |
| The biosynthesis of thiamine. 2. Fractionation of enzyme system and identification of thiazole monophosphate and thiamine monophosphate as intermediates. | CAMIENER GW, BROWN GM | 1960 Aug | 13807175 |
| Systematic discovery of analogous enzymes in thiamin biosynthesis. | Morett E, Korbel JO, Rajan E, Saab-Rincon G, Olvera L, Olvera M, Schmidt S, Snel B, Bork P | 2003 Jul | 12794638 |
| Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution. | Chiu HJ, Reddick JJ, Begley TP, Ealick SE | 1999 May 18 | 10350464 |
| RHEA:22328 | 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[O;H0;+0:3]-[*:4].[*:5]:[n;H0;+0:6]:[*:7].[H+;H0:8]>>[*:1]-[CH2;+0:2]-[n+;H0:6](:[*:5]):[*:7].[*:4]-[OH;+0:3] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| A Brassica cDNA clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis. | Kim YS, Nosaka K, Downs DM, Kwak JM, Park D, Chung IK, Nam HG | 1998 Aug | 9700068 |
| Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis. | Zhang Y, Taylor SV, Chiu HJ, Begley TP | 1997 May | 9139923 |
| Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway. | Kawasaki Y | 1993 Aug | 8394314 |
| Identification of inhibitors against Mycobacterium tuberculosis thiamin phosphate synthase, an important target for the development of anti-TB drugs. | Khare G, Kar R, Tyagi AK | 2011 | 21818324 |
| Determination of the genetic, molecular, and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1. | Ajjawi I, Tsegaye Y, Shintani D | 2007 Mar 1 | 17174261 |
| A th-1 Mutant of Arabidopsis thaliana Is Defective for a Thiamin-Phosphate-Synthesizing Enzyme: Thiamin Phosphate Pyrophosphorylase. | Komeda Y, Tanaka M, Nishimune T | 1988 Oct | 16666289 |
| The enzymatic synthesis of thiamine monophosphate. | LEDER IG | 1961 Nov | 14463407 |
| The biosynthesis of thiamine. 2. Fractionation of enzyme system and identification of thiazole monophosphate and thiamine monophosphate as intermediates. | CAMIENER GW, BROWN GM | 1960 Aug | 13807175 |
| Systematic discovery of analogous enzymes in thiamin biosynthesis. | Morett E, Korbel JO, Rajan E, Saab-Rincon G, Olvera L, Olvera M, Schmidt S, Snel B, Bork P | 2003 Jul | 12794638 |
| Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution. | Chiu HJ, Reddick JJ, Begley TP, Ealick SE | 1999 May 18 | 10350464 |