EC number:2.8.1.4
Enzyme
Download| EC Tree |
| 2. Transferases |
| 2.8 Transferring sulfur-containing groups |
| 2.8.1 Sulfurtransferases |
| ID: | 2.8.1.4 |
|---|---|
| Description: | tRNA uracil 4-sulfurtransferase. |
| Alternative Name: |
tRNA sulfurtransferase. |
| Cath: | 3.30.2130.30; 3.40.50.620; |
3D structure
Click one PDB to see exact 3D structure provided by NGL.
Note: Use your mouse to drag, rotate, and zoom in and out of the structure. Mouse-over to identify atoms and bonds. Mouse controls documentation.References
External Links
| UniProtKB Enzyme Link: | UniProtKB 2.8.1.4 |
| BRENDA Enzyme Link: | BRENDA 2.8.1.4 |
| KEGG Enzyme Link: | KEGG2.8.1.4 |
| BioCyc Enzyme Link: | BioCyc 2.8.1.4 |
| ExPASy Enzyme Link: | ExPASy2.8.1.4 |
| EC2PDB Enzyme Link: | EC2PDB 2.8.1.4 |
| ExplorEnz Enzyme Link: | ExplorEnz 2.8.1.4 |
| PRIAM enzyme-specific profiles Link: | PRIAM 2.8.1.4 |
| IntEnz Enzyme Link: | IntEnz 2.8.1.4 |
| MEDLINE Enzyme Link: | MEDLINE 2.8.1.4 |
| RHEA:24176 | [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] |
| RULE(radius=1) | [*:1]-[Fe;H0;+0:2]-[*:3].[*:4]-[Fe;H0;+0:5]-[*:6].[*:7]-[O;H0;+0:8]-[P;H0;+0:9](-[*:10])(=[*:11])-[*:12].[*:13]-[S;H0;+0:14]-[SH;+0:15].[*:16]:[c;H0;+0:17](:[*:18])=[O;H0;+0:19].[H+;H0:20]>>[*:1]-[Fe+;H0:2]-[*:3].[*:4]-[Fe+;H0:5]-[*:6].[*:7]-[OH;+0:8].[*:10]-[P;H0;+0:9](=[*:11])(-[*:12])-[OH;+0:19].[*:13]-[SH;+0:14].[*:16]:[c;H0;+0:17](:[*:18])=[S;H0;+0:15] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| The biosynthesis of 4-thiouridylate. Separation and purification of two enzymes in the transfer ribonucleic acid-sulfurtransferase system. | Abrell JW, Kaufman EE, Lipsett MN | 1971 Jan 25 | 5541999 |
| Enzymatic thiolation of E. coli sRNA. | Lipsett MN, Peterkofsky A | 1966 May | 5334201 |
| RNA thiolase: the enzymatic transfer of sulfur from cysteine to sRNA in Escherichia coli extracts. | Hayward RS, Weiss SB | 1966 May | 5334200 |
| Ribonucleic acid sulfurtransferase from Bacillus subtilis W168. Sulfuration with beta-mercaptopyruvate and properties of the enzyme system. | Wong TW, Weiss SB, Eliceiri GL, Bryant J | 1970 May 26 | 4987417 |
| A [3Fe-4S] cluster is required for tRNA thiolation in archaea and eukaryotes. | Liu Y, Vinyard DJ, Reesbeck ME, Suzuki T, Manakongtreecheep K, Holland PL, Brudvig GW, Söll D | 2016 Nov 8 | 27791189 |
| Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification. | Neumann P, Lakomek K, Naumann PT, Erwin WM, Lauhon CT, Ficner R | 2014 Jun | 24705700 |
| Substrate specificity for 4-thiouridine modification in Escherichia coli. | Lauhon CT, Erwin WM, Ton GN | 2004 May 28 | 15037613 |
| The role of the cysteine residues of ThiI in the generation of 4-thiouridine in tRNA. | Mueller EG, Palenchar PM, Buck CJ | 2001 Sep 7 | 11443125 |
| Evidence for the transfer of sulfane sulfur from IscS to ThiI during the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA. | Kambampati R, Lauhon CT | 2000 Apr 14 | 10753862 |