Enzyme

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     4. Lyases
        4.2 Carbon-oxygen lyases
            4.2.99 Other carbon-oxygen lyases
ID:4.2.99.21
Description:Isochorismate lyase.
Alternative Name: Isochorismate pyruvate lyase.
Cath: 3.60.120.10; 1.20.59.10;

3D structure

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References

External Links

UniProtKB Enzyme Link: UniProtKB 4.2.99.21
BRENDA Enzyme Link: BRENDA 4.2.99.21
KEGG Enzyme Link: KEGG4.2.99.21
BioCyc Enzyme Link: BioCyc 4.2.99.21
ExPASy Enzyme Link: ExPASy4.2.99.21
EC2PDB Enzyme Link: EC2PDB 4.2.99.21
ExplorEnz Enzyme Link: ExplorEnz 4.2.99.21
PRIAM enzyme-specific profiles Link: PRIAM 4.2.99.21
IntEnz Enzyme Link: IntEnz 4.2.99.21
MEDLINE Enzyme Link: MEDLINE 4.2.99.21
MSA:

4.2.99.21;

Phylogenetic Tree:

4.2.99.21;

Uniprot:
M-CSA:
RHEA:27874 isochorismate = pyruvate + salicylate
RULE(radius=1) [*:1]-[C;H0;+0:2]1=[CH;+0:3]-[CH;+0:4]=[CH;+0:5]-[CH;+0:6](-[O;H0;+0:7]-[C;H0;+0:8](-[*:9])=[CH2;+0:10])-[CH;+0:11]-1-[*:12]>>[*:9]-[C;H0;+0:8](-[CH3;+0:10])=[O;H0;+0:7].[*:1]-[c;H0;+0:2]1:[cH;+0:3]:[cH;+0:4]:[cH;+0:5]:[cH;+0:6]:[c;H0;+0:11]:1-[*:12]
Reaction
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References

TitleAuthorsDatePubMed ID
Structure and mechanism of MbtI, the salicylate synthase from Mycobacterium tuberculosis.Zwahlen J, Kolappan S, Zhou R, Kisker C, Tonge PJ2007 Jan 3017240979
Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa.Serino L, Reimmann C, Baur H, Beyeler M, Visca P, Haas D1995 Nov 157500944
Modification of residue 42 of the active site loop with a lysine-mimetic side chain rescues isochorismate-pyruvate lyase activity in Pseudomonas aeruginosa PchB.Olucha J, Meneely KM, Lamb AL2012 Sep 2522970849
Pericyclic reactions catalyzed by chorismate-utilizing enzymes.Lamb AL2011 Sep 621823653
pH Dependence of catalysis by Pseudomonas aeruginosa isochorismate-pyruvate lyase: implications for transition state stabilization and the role of lysine 42.Olucha J, Ouellette AN, Luo Q, Lamb AL2011 Aug 2321751784
Entropic and enthalpic components of catalysis in the mutase and lyase activities of Pseudomonas aeruginosa PchB.Luo Q, Meneely KM, Lamb AL2011 May 1121504201
Inhibition studies of Mycobacterium tuberculosis salicylate synthase (MbtI).Manos-Turvey A, Bulloch EM, Rutledge PJ, Baker EN, Lott JS, Payne RJ2010 Jul 520512795
The structure of MbtI from Mycobacterium tuberculosis, the first enzyme in the biosynthesis of the siderophore mycobactin, reveals it to be a salicylate synthase.Harrison AJ, Yu M, Gårdenborg T, Middleditch M, Ramsay RJ, Baker EN, Lott JS2006 Sep16923875
Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection.Künzler DE, Sasso S, Gamper M, Hilvert D, Kast P2005 Sep 2316036918
Salicylate biosynthesis: overexpression, purification, and characterization of Irp9, a bifunctional salicylate synthase from Yersinia enterocolitica.Kerbarh O, Ciulli A, Howard NI, Abell C2005 Aug16030197