EC number:1.14.18.8

Enzyme

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     1. Oxidoreductases
        1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
            1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
ID:1.14.18.8
Description:7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase.
Alternative Name: Sterol 12-alpha-hydroxylase.
HCO 12-alpha-hydroxylase.
CYP12.
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-monooxygenase.
Prosite: PDOC00081;
PDB:
PDBScop

3D structure

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PDB

References

External Links

UniProtKB Enzyme Link: UniProtKB 1.14.18.8
BRENDA Enzyme Link: BRENDA 1.14.18.8
KEGG Enzyme Link: KEGG1.14.18.8
BioCyc Enzyme Link: BioCyc 1.14.18.8
ExPASy Enzyme Link: ExPASy1.14.18.8
EC2PDB Enzyme Link: EC2PDB 1.14.18.8
ExplorEnz Enzyme Link: ExplorEnz 1.14.18.8
PRIAM enzyme-specific profiles Link: PRIAM 1.14.18.8
IntEnz Enzyme Link: IntEnz 1.14.18.8
MEDLINE Enzyme Link: MEDLINE 1.14.18.8

EC number:1.14.18.8

MSA:

1.14.18.8;
Phylogenetic Tree:

1.14.18.8;
Uniprot:
M-CSA:
RHEA:46752 7alpha-hydroxycholest-4-en-3-one + 2 [Fe(II)-cytochrome b5] + 2 H(+) + O2 = 7alpha,12alpha-dihydroxycholest-4-en-3-one + 2 [Fe(III)-cytochrome b5] + H2O
RULE(radius=1) [*:1]-[CH2;+0:2]-[*:3].[Fe+2;H0:4].[Fe+2;H0:5].[H+;H0:6].[H+;H0:7].[O;H0;+0:8]=[O;H0;+0:9]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:8].[Fe+3;H0:4].[Fe+3;H0:5].[OH2;+0:9]
Reaction
Core-to-Core No scaffolds atoms were exchanged as a result of the reaction

References

TitleAuthorsDatePubMed ID
The enzymes, regulation, and genetics of bile acid synthesis.Russell DW200312543708
Molecular cloning and expression of rabbit sterol 12alpha-hydroxylase.Eggertsen G, Olin M, Andersson U, Ishida H, Kubota S, Hellman U, Okuda KI, Björkhem I1996 Dec 138943286
Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase.Ishida H, Noshiro M, Okuda K, Coon MJ1992 Oct 251400444