EC number:1.14.19.29
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water |
| ID: | 1.14.19.29 |
|---|---|
| Description: | Sphingolipid 8-(E/Z)-desaturase. |
| Alternative Name: |
8-sphingolipid desaturase. 8 fatty acid desaturase. |
3D structure
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Note: Use your mouse to drag, rotate, and zoom in and out of the structure. Mouse-over to identify atoms and bonds. Mouse controls documentation.References
External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.19.29 |
| BRENDA Enzyme Link: | BRENDA 1.14.19.29 |
| KEGG Enzyme Link: | KEGG1.14.19.29 |
| BioCyc Enzyme Link: | BioCyc 1.14.19.29 |
| ExPASy Enzyme Link: | ExPASy1.14.19.29 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.19.29 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.19.29 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.19.29 |
| IntEnz Enzyme Link: | IntEnz 1.14.19.29 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.19.29 |
EC number:1.14.19.29
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:46272 | 2 [Fe(II)-cytochrome b5] + 2 H(+) + N-acyl-(4R)-hydroxysphinganine + O2 = (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 [Fe(III)-cytochrome b5] + 2 H2O |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[CH2;+0:3]-[*:4].[Fe+2;H0:5].[Fe+2;H0:6].[H+;H0:7].[H+;H0:8].[O;H0;+0:9]=[O;H0;+0:10]>>[*:1]-[CH;+0:2]=[CH;+0:3]-[*:4].[Fe+3;H0:5].[Fe+3;H0:6].[OH2;+0:9].[OH2;+0:10] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Conformational studies on the Delta8(E,Z)-sphingolipid desaturase from Helianthus annuus with chiral fluoropalmitic acids as mechanistic probes. | Habel A, Sperling P, Bartram S, Heinz E, Boland W | 2010 Aug 6 | 20575587 |
| Disruption of the sphingolipid Delta8-desaturase gene causes a delay in morphological changes in Candida albicans. | Oura T, Kajiwara S | 2008 Dec | 19047747 |
| A higher plant delta8 sphingolipid desaturase with a preference for (Z)-isomer formation confers aluminum tolerance to yeast and plants. | Ryan PR, Liu Q, Sperling P, Dong B, Franke S, Delhaize E | 2007 Aug | 17600137 |
| A sphingolipid desaturase from higher plants. Identification of a new cytochrome b5 fusion protein. | Sperling P, Zähringer U, Heinz E | 1998 Oct 30 | 9786850 |
| Sphingolipid Δ8 unsaturation is important for glucosylceramide biosynthesis and low-temperature performance in Arabidopsis. | Chen M, Markham JE, Cahoon EB | 2012 Mar | 22023480 |
| Fatty acid desaturases from the microalga Thalassiosira pseudonana. | Tonon T, Sayanova O, Michaelson LV, Qing R, Harvey D, Larson TR, Li Y, Napier JA, Graham IA | 2005 Jul | 15978045 |
| Isolation and characterization of the genes encoding delta(8)-sphingolipid desaturase from Saccharomyces kluyveri and Kluyveromyces lactis. | Takakuwa N, Kinoshita M, Oda Y, Ohnishi M | 2002 Dec | 12402089 |
| Characterization of a Delta8-sphingolipid desaturase from higher plants: a stereochemical and mechanistic study on the origin of E,Z isomers. | Beckmann C, Rattke J, Oldham NJ, Sperling P, Heinz E, Boland W | 2002 Jul 2 | 12203571 |
| Functional identification of a delta8-sphingolipid desaturase from Borago officinalis. | Sperling P, Libisch B, Zähringer U, Napier JA, Heinz E | 2001 Apr 15 | 11368168 |
| Further characterization of Delta(8)-sphingolipid desaturases from higher plants. | Sperling P, Blume A, Zähringer U, Heinz E | 2000 Dec | 11171153 |
| The Delta8-desaturase of Euglena gracilis: an alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids. | Wallis JG, Browse J | 1999 May 15 | 10328826 |
| RHEA:46268 | 2 [Fe(II)-cytochrome b5] + 2 H(+) + N-acyl-(4R)-hydroxysphinganine + O2 = (4R,8E)-4-hydroxysphingenine ceramide + 2 [Fe(III)-cytochrome b5] + 2 H2O |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[CH2;+0:3]-[*:4].[Fe+2;H0:5].[Fe+2;H0:6].[H+;H0:7].[H+;H0:8].[O;H0;+0:9]=[O;H0;+0:10]>>[*:1]-[CH;+0:2]=[CH;+0:3]-[*:4].[Fe+3;H0:5].[Fe+3;H0:6].[OH2;+0:9].[OH2;+0:10] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Conformational studies on the Delta8(E,Z)-sphingolipid desaturase from Helianthus annuus with chiral fluoropalmitic acids as mechanistic probes. | Habel A, Sperling P, Bartram S, Heinz E, Boland W | 2010 Aug 6 | 20575587 |
| Disruption of the sphingolipid Delta8-desaturase gene causes a delay in morphological changes in Candida albicans. | Oura T, Kajiwara S | 2008 Dec | 19047747 |
| A higher plant delta8 sphingolipid desaturase with a preference for (Z)-isomer formation confers aluminum tolerance to yeast and plants. | Ryan PR, Liu Q, Sperling P, Dong B, Franke S, Delhaize E | 2007 Aug | 17600137 |
| A sphingolipid desaturase from higher plants. Identification of a new cytochrome b5 fusion protein. | Sperling P, Zähringer U, Heinz E | 1998 Oct 30 | 9786850 |
| Sphingolipid Δ8 unsaturation is important for glucosylceramide biosynthesis and low-temperature performance in Arabidopsis. | Chen M, Markham JE, Cahoon EB | 2012 Mar | 22023480 |
| Fatty acid desaturases from the microalga Thalassiosira pseudonana. | Tonon T, Sayanova O, Michaelson LV, Qing R, Harvey D, Larson TR, Li Y, Napier JA, Graham IA | 2005 Jul | 15978045 |
| Isolation and characterization of the genes encoding delta(8)-sphingolipid desaturase from Saccharomyces kluyveri and Kluyveromyces lactis. | Takakuwa N, Kinoshita M, Oda Y, Ohnishi M | 2002 Dec | 12402089 |
| Characterization of a Delta8-sphingolipid desaturase from higher plants: a stereochemical and mechanistic study on the origin of E,Z isomers. | Beckmann C, Rattke J, Oldham NJ, Sperling P, Heinz E, Boland W | 2002 Jul 2 | 12203571 |
| Functional identification of a delta8-sphingolipid desaturase from Borago officinalis. | Sperling P, Libisch B, Zähringer U, Napier JA, Heinz E | 2001 Apr 15 | 11368168 |
| Further characterization of Delta(8)-sphingolipid desaturases from higher plants. | Sperling P, Blume A, Zähringer U, Heinz E | 2000 Dec | 11171153 |
| The Delta8-desaturase of Euglena gracilis: an alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids. | Wallis JG, Browse J | 1999 May 15 | 10328826 |