EC number:1.14.19.6
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water |
| ID: | 1.14.19.6 | ||
|---|---|---|---|
| Description: | Acyl-CoA (9+3)-desaturase. | ||
| Alternative Name: |
Oleoyl-CoA Delta(12) desaturase. Oleoyl-CoA 12-desaturase. Delta(12)-fatty-acid desaturase. Delta(12)(omega(6))-desaturase. Delta(12) fatty acid desaturase. Delta(12) desaturase. | ||
| Prosite: | PDOC00399; | ||
| PDB: |
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3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.19.6 |
| BRENDA Enzyme Link: | BRENDA 1.14.19.6 |
| KEGG Enzyme Link: | KEGG1.14.19.6 |
| BioCyc Enzyme Link: | BioCyc 1.14.19.6 |
| ExPASy Enzyme Link: | ExPASy1.14.19.6 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.19.6 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.19.6 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.19.6 |
| IntEnz Enzyme Link: | IntEnz 1.14.19.6 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.19.6 |
EC number:1.14.19.6
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:45096 | (9Z)-hexadecenoyl-CoA + 2 [Fe(II)-cytochrome b5] + 2 H(+) + O2 = (9Z,12Z)-hexadecadienoyl-CoA + 2 [Fe(III)-cytochrome b5] + 2 H2O |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[CH2;+0:3]-[*:4].[Fe+2;H0:5].[Fe+2;H0:6].[H+;H0:7].[H+;H0:8].[O;H0;+0:9]=[O;H0;+0:10]>>[*:1]-[CH;+0:2]=[CH;+0:3]-[*:4].[Fe+3;H0:5].[Fe+3;H0:6].[OH2;+0:9].[OH2;+0:10] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Recent advances in the biochemistry and molecular biology of fatty acyl desaturases. | Tocher DR, Leaver MJ, Hodgson PA | 1998 Jul-Aug | 9829122 |
| Study of the delta 12-desaturase system of Lipomyces starkeyi. | Lomascolo A, Dubreucq E, Galzy P | 1996 Mar | 8900454 |
| Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae. | Covello PS, Reed DW | 1996 May | 8685264 |
| Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate. | Borgeson CE, de Renobales M, Blomquist GJ | 1990 Nov 12 | 2248971 |
| Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. | Wada H, Gombos Z, Murata N | 1990 Sep 13 | 2118597 |
| The expression of three desaturase genes of Spirulina platensis in Escherichia coli DH5alpha. Heterologous expression of Spirulina-desaturase genes. | Apiradee H, Kalyanee P, Pongsathon P, Patcharaporn D, Matura S, Sanjukta S, Supapon C, Morakot T | 2004 Sep | 15560373 |
| Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity. | Dyer JM, Chapital DC, Kuan JC, Mullen RT, Turner C, McKeon TA, Pepperman AB | 2002 Dec | 12481086 |
| Cloning of delta12- and delta6-desaturases from Mortierella alpina and recombinant production of gamma-linolenic acid in Saccharomyces cerevisiae. | Huang YS, Chaudhary S, Thurmond JM, Bobik EG Jr, Yuan L, Chan GM, Kirchner SJ, Mukerji P, Knutzon DS | 1999 Jul | 10478922 |
| Identification of Delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae. | Sakuradani E, Kobayashi M, Ashikari T, Shimizu S | 1999 May | 10215899 |
| RHEA:25856 | (9Z)-octadecenoyl-CoA + 2 [Fe(II)-cytochrome b5] + 2 H(+) + O2 = (9Z,12Z)-octadecadienoyl-CoA + 2 [Fe(III)-cytochrome b5] + 2 H2O |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[CH2;+0:3]-[*:4].[Fe+2;H0:5].[Fe+2;H0:6].[H+;H0:7].[H+;H0:8].[O;H0;+0:9]=[O;H0;+0:10]>>[*:1]-[CH;+0:2]=[CH;+0:3]-[*:4].[Fe+3;H0:5].[Fe+3;H0:6].[OH2;+0:9].[OH2;+0:10] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Recent advances in the biochemistry and molecular biology of fatty acyl desaturases. | Tocher DR, Leaver MJ, Hodgson PA | 1998 Jul-Aug | 9829122 |
| Study of the delta 12-desaturase system of Lipomyces starkeyi. | Lomascolo A, Dubreucq E, Galzy P | 1996 Mar | 8900454 |
| Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae. | Covello PS, Reed DW | 1996 May | 8685264 |
| Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate. | Borgeson CE, de Renobales M, Blomquist GJ | 1990 Nov 12 | 2248971 |
| Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. | Wada H, Gombos Z, Murata N | 1990 Sep 13 | 2118597 |
| The expression of three desaturase genes of Spirulina platensis in Escherichia coli DH5alpha. Heterologous expression of Spirulina-desaturase genes. | Apiradee H, Kalyanee P, Pongsathon P, Patcharaporn D, Matura S, Sanjukta S, Supapon C, Morakot T | 2004 Sep | 15560373 |
| Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity. | Dyer JM, Chapital DC, Kuan JC, Mullen RT, Turner C, McKeon TA, Pepperman AB | 2002 Dec | 12481086 |
| Cloning of delta12- and delta6-desaturases from Mortierella alpina and recombinant production of gamma-linolenic acid in Saccharomyces cerevisiae. | Huang YS, Chaudhary S, Thurmond JM, Bobik EG Jr, Yuan L, Chan GM, Kirchner SJ, Mukerji P, Knutzon DS | 1999 Jul | 10478922 |
| Identification of Delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae. | Sakuradani E, Kobayashi M, Ashikari T, Shimizu S | 1999 May | 10215899 |