Enzyme

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     1. Oxidoreductases
        1.7 Acting on other nitrogenous compounds as donors
            1.7.1 With NAD+ or NADP+ as acceptor
ID:1.7.1.1
Description:Nitrate reductase (NADH).
Alternative Name: Nitrate reductase (NADH(2)).
NADH:nitrate oxidoreductase.
NADH-nitrate reductase.
NADH-dependent nitrate reductase.
Assimilatory nitrate reductase.
Assimilatory NADH:nitrate reductase.
Prosite: PDOC00484; PDOC00170;
PDB:
PDBScop
3NS1 8030683; 8043062; 8030683; 8043062; 8030678; 8030679; 8043057; 8043058; 8030678; 8030679; 8043057; 8043058; 8030682; 8043061; 8030682; 8043061; 8030680; 8043059; 8030680; 8043059; 8030686; 8043065; 8030686; 8043065;
1FIQ 8030683; 8043062; 8030678; 8030679; 8043057; 8043058; 8030682; 8043061; 8030680; 8043059; 8030686; 8043065;
3NVY 8030682; 8043061; 8030683; 8043062; 8030683; 8043062; 8030678; 8030679; 8043057; 8043058; 8030678; 8030679; 8043057; 8043058; 8030682; 8030686; 8043061; 8043065; 8030686; 8043065; 8030680; 8043059; 8030680; 8043059;
3ETR 8030682; 8043061; 8030678; 8030679; 8043057; 8043058; 8030683; 8043062; 8030682; 8043061; 8030683; 8043062; 8030678; 8030679; 8043057; 8043058; 8030686; 8043065; 8030680; 8043059; 8030686; 8043065; 8030680; 8043059;
3EUB 8030678; 8030679; 8043057; 8043058; 8030682; 8043061; 8030683; 8043062; 8030682; 8043061; 8030682; 8043061; 8030683; 8043062; 8030683; 8043062; 8030683; 8043062; 8030678; 8030679; 8043057; 8043058; 8030678; 8030679; 8043057; 8043058; 8030682; 8043061; 8030678; 8030679; 8043057; 8043058; 8030686; 8043065; 8030680; 8043059; 8030686; 8043065; 8030686; 8043065; 8030680; 8043059; 8030680; 8043059; 8030680; 8043059; 8030686; 8043065;
 » show all

Cath: 3.40.50.80; 2.40.30.10;

3D structure

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References

External Links

UniProtKB Enzyme Link: UniProtKB 1.7.1.1
BRENDA Enzyme Link: BRENDA 1.7.1.1
KEGG Enzyme Link: KEGG1.7.1.1
BioCyc Enzyme Link: BioCyc 1.7.1.1
ExPASy Enzyme Link: ExPASy1.7.1.1
EC2PDB Enzyme Link: EC2PDB 1.7.1.1
ExplorEnz Enzyme Link: ExplorEnz 1.7.1.1
PRIAM enzyme-specific profiles Link: PRIAM 1.7.1.1
IntEnz Enzyme Link: IntEnz 1.7.1.1
MEDLINE Enzyme Link: MEDLINE 1.7.1.1
MSA:

1.7.1.1;

Phylogenetic Tree:

1.7.1.1;

Uniprot:
M-CSA:
RHEA:17913 H2O + NAD(+) + nitrite = H(+) + NADH + nitrate
RULE(radius=1) [*:1]-[n+;H0:2]1:[cH;+0:3]:[cH;+0:4]:[cH;+0:5]:[c;H0;+0:6](-[*:7]):[cH;+0:8]:1.[*:9]=[N;H0;+0:10]-[*:11].[OH2;+0:12]>>[*:1]-[N;H0;+0:2]1-[CH;+0:3]=[CH;+0:4]-[CH2;+0:5]-[C;H0;+0:6](-[*:7])=[CH;+0:8]-1.[*:9]=[N+;H0:10](-[*:11])-[OH;+0:12]
Reaction
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References

TitleAuthorsDatePubMed ID
Differential expression of the arabidopsis nia1 and nia2 genes. cytokinin-induced nitrate reductase activity is correlated with increased nia1 transcription and mrna levels Yu X, Sukumaran S, Mrton L1998 Mar9501142
Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions.Berks BC, Ferguson SJ, Moir JW, Richardson DJ1995 Dec 128534676
Kinetic mechanism of assimilatory NADH:nitrate reductase from Chlorella.Howard WD, Solomonson LP1981 Dec 256273405
Purification and Kinetics of Higher Plant NADH:Nitrate Reductase.Campbell WH, Smarrelli J1978 Apr16660347
Pre-steady-state kinetic analysis of recombinant Arabidopsis NADH:nitrate reductase: rate-limiting processes in catalysis.Skipper L, Campbell WH, Mertens JA, Lowe DJ2001 Jul 2011356830
Structure and function of eukaryotic NAD(P)H:nitrate reductase.Campbell WH2001 Feb11289301