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2. Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

ID:	2.1.1.260
Description:	rRNA small subunit pseudouridine methyltransferase Nep1.
Cath:	3.40.1280.10;

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UniProtKB Enzyme Link:	UniProtKB 2.1.1.260
BRENDA Enzyme Link:	BRENDA 2.1.1.260
KEGG Enzyme Link:	KEGG2.1.1.260
BioCyc Enzyme Link:	BioCyc 2.1.1.260
ExPASy Enzyme Link:	ExPASy2.1.1.260
EC2PDB Enzyme Link:	EC2PDB 2.1.1.260
ExplorEnz Enzyme Link:	ExplorEnz 2.1.1.260
PRIAM enzyme-specific profiles Link:	PRIAM 2.1.1.260
IntEnz Enzyme Link:	IntEnz 2.1.1.260
MEDLINE Enzyme Link:	MEDLINE 2.1.1.260

RHEA:54308	pseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-homocysteine
RULE(radius=1)	[:1]-[S+;H0:2](-[:3])-[CH3;+0:4].[:5]:[nH;+0:6]:[:7]>>[:1]-[S;H0;+0:2]-[:3].[:5]:[n;H0;+0:6](:[:7])-[CH3;+0:4]
Reaction
Core-to-Core	No scaffolds atoms were exchanged as a result of the reaction

Title	Authors	Date	PubMed ID
The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA.	Meyer B, Wurm JP, Kötter P, Leisegang MS, Schilling V, Buchhaupt M, Held M, Bahr U, Karas M, Heckel A, Bohnsack MT, Wöhnert J, Entian KD	2011 Mar	20972225
The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site.	Taylor AB, Meyer B, Leal BZ, Kötter P, Schirf V, Demeler B, Hart PJ, Entian KD, Wöhnert J	2008 Mar	18208838

RHEA:46716	pseudouridine(914) in M. jannaschii 16S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(914) in M. jannaschii 16S rRNA + S-adenosyl-L-homocysteine
RULE(radius=1)	[:1]-[S+;H0:2](-[:3])-[CH3;+0:4].[:5]:[nH;+0:6]:[:7]>>[:1]-[S;H0;+0:2]-[:3].[:5]:[n;H0;+0:6](:[:7])-[CH3;+0:4]
Reaction
Core-to-Core	No scaffolds atoms were exchanged as a result of the reaction

Title	Authors	Date	PubMed ID
The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.	Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kötter P, Engels JW, Heckel A, Karas M, Entian KD, Wöhnert J	2010 Apr	20047967

RHEA:46712	pseudouridine(1248) in human 18S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(1248) in human 18S rRNA + S-adenosyl-L-homocysteine
RULE(radius=1)	[:1]-[S+;H0:2](-[:3])-[CH3;+0:4].[:5]:[nH;+0:6]:[:7]>>[:1]-[S;H0;+0:2]-[:3].[:5]:[n;H0;+0:6](:[:7])-[CH3;+0:4]
Reaction
Core-to-Core	No scaffolds atoms were exchanged as a result of the reaction

Title	Authors	Date	PubMed ID
The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.	Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kötter P, Engels JW, Heckel A, Karas M, Entian KD, Wöhnert J	2010 Apr	20047967

RHEA:46696	a pseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L-homocysteine
RULE(radius=1)	[:1]-[S+;H0:2](-[:3])-[CH3;+0:4].[:5]:[nH;+0:6]:[:7]>>[:1]-[S;H0;+0:2]-[:3].[:5]:[n;H0;+0:6](:[:7])-[CH3;+0:4]
Reaction
Core-to-Core	No scaffolds atoms were exchanged as a result of the reaction

Title	Authors	Date	PubMed ID
The Schizosaccharomyces pombe mra1 gene, which is required for cell growth and mating, can suppress the mating inefficiency caused by a deficit in the Ras1 activity.	Hakuno F, Hughes DA, Yamamoto M	1996 Mar	9133664