EC number:2.8.4.4
Enzyme
Download| EC Tree |
| 2. Transferases |
| 2.8 Transferring sulfur-containing groups |
| 2.8.4 Transferring alkylthio groups |
| ID: | 2.8.4.4 |
|---|---|
| Description: | [Ribosomal protein S12] (aspartate(89)-C(3))-methylthiotransferase. |
| Alternative Name: |
[Ribosomal protein S12] (aspartate-C(3))-methylthiotransferase. |
| Cath: | 3.30.750.200; 3.30.750.210; 3.80.30.20; 2.40.50.140; 3.40.50.12160; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 2.8.4.4 |
| BRENDA Enzyme Link: | BRENDA 2.8.4.4 |
| KEGG Enzyme Link: | KEGG2.8.4.4 |
| BioCyc Enzyme Link: | BioCyc 2.8.4.4 |
| ExPASy Enzyme Link: | ExPASy2.8.4.4 |
| EC2PDB Enzyme Link: | EC2PDB 2.8.4.4 |
| ExplorEnz Enzyme Link: | ExplorEnz 2.8.4.4 |
| PRIAM enzyme-specific profiles Link: | PRIAM 2.8.4.4 |
| IntEnz Enzyme Link: | IntEnz 2.8.4.4 |
| MEDLINE Enzyme Link: | MEDLINE 2.8.4.4 |
| RHEA:37083 | [sulfur carrier]-SH + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + S-adenosyl-L-methionine = [sulfur carrier]-H + H(+) + L-aspartate(89)-[ribosomal protein uS12]-methanesulfate + S-adenosyl-L-homocysteine |
| RULE(radius=1) | [*:1]-[S+;H0:2](-[*:3])-[CH3;+0:4].[*:5]-[SH;+0:6]>>[*:1]-[S;H0;+0:2]-[*:3].[*:5]-[S;H0;+0:6]-[CH3;+0:4] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Identification of an intermediate methyl carrier in the radical S-adenosylmethionine methylthiotransferases RimO and MiaB. | Landgraf BJ, Arcinas AJ, Lee KH, Booker SJ | 2013 Oct 16 | 23991893 |
| Two Fe-S clusters catalyze sulfur insertion by radical-SAM methylthiotransferases. | Forouhar F, Arragain S, Atta M, Gambarelli S, Mouesca JM, Hussain M, Xiao R, Kieffer-Jaquinod S, Seetharaman J, Acton TB, Montelione GT, Mulliez E, Hunt JF, Fontecave M | 2013 May | 23542644 |
| A proteomic and transcriptomic approach reveals new insight into beta-methylthiolation of Escherichia coli ribosomal protein S12. | Strader MB, Costantino N, Elkins CA, Chen CY, Patel I, Makusky AJ, Choy JS, Court DL, Markey SP, Kowalak JA | 2011 Mar | 21169565 |
| Post-translational modification of ribosomal proteins: structural and functional characterization of RimO from Thermotoga maritima, a radical S-adenosylmethionine methylthiotransferase. | Arragain S, Garcia-Serres R, Blondin G, Douki T, Clemancey M, Latour JM, Forouhar F, Neely H, Montelione GT, Hunt JF, Mulliez E, Fontecave M, Atta M | 2010 Feb 19 | 20007320 |
| Characterization of RimO, a new member of the methylthiotransferase subclass of the radical SAM superfamily. | Lee KH, Saleh L, Anton BP, Madinger CL, Benner JS, Iwig DF, Roberts RJ, Krebs C, Booker SJ | 2009 Oct 27 | 19736993 |
| RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli. | Anton BP, Saleh L, Benner JS, Raleigh EA, Kasif S, Roberts RJ | 2008 Feb 12 | 18252828 |
| RHEA:36351 | AH2 + L-aspartate(89)-[ribosomal protein uS12]-methanesulfate + S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + A + H(+) + L-methionine |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[*:3].[*:4]-[CH2;+0:5]-[S+;H0:6](-[*:7])-[*:8].[*:9]-[S;H0;+0:10]-[*;H0;+0:11]>>[*;H0;+0:11].[*:4]-[CH3;+0:5].[*:1]-[CH;+0:2](-[*:3])-[S;H0;+0:10]-[*:9].[*:7]-[S;H0;+0:6]-[*:8] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Identification of an intermediate methyl carrier in the radical S-adenosylmethionine methylthiotransferases RimO and MiaB. | Landgraf BJ, Arcinas AJ, Lee KH, Booker SJ | 2013 Oct 16 | 23991893 |
| Two Fe-S clusters catalyze sulfur insertion by radical-SAM methylthiotransferases. | Forouhar F, Arragain S, Atta M, Gambarelli S, Mouesca JM, Hussain M, Xiao R, Kieffer-Jaquinod S, Seetharaman J, Acton TB, Montelione GT, Mulliez E, Hunt JF, Fontecave M | 2013 May | 23542644 |
| A proteomic and transcriptomic approach reveals new insight into beta-methylthiolation of Escherichia coli ribosomal protein S12. | Strader MB, Costantino N, Elkins CA, Chen CY, Patel I, Makusky AJ, Choy JS, Court DL, Markey SP, Kowalak JA | 2011 Mar | 21169565 |
| Post-translational modification of ribosomal proteins: structural and functional characterization of RimO from Thermotoga maritima, a radical S-adenosylmethionine methylthiotransferase. | Arragain S, Garcia-Serres R, Blondin G, Douki T, Clemancey M, Latour JM, Forouhar F, Neely H, Montelione GT, Hunt JF, Mulliez E, Fontecave M, Atta M | 2010 Feb 19 | 20007320 |
| Characterization of RimO, a new member of the methylthiotransferase subclass of the radical SAM superfamily. | Lee KH, Saleh L, Anton BP, Madinger CL, Benner JS, Iwig DF, Roberts RJ, Krebs C, Booker SJ | 2009 Oct 27 | 19736993 |
| RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli. | Anton BP, Saleh L, Benner JS, Raleigh EA, Kasif S, Roberts RJ | 2008 Feb 12 | 18252828 |