EC number:2.8.4.5

Enzyme

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     2. Transferases
        2.8 Transferring sulfur-containing groups
            2.8.4 Transferring alkylthio groups
ID:2.8.4.5
Description:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase.
Alternative Name: Methylthio-threonylcarbamoyl-adenosine transferase B.

3D structure

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PDB

References

External Links

UniProtKB Enzyme Link: UniProtKB 2.8.4.5
BRENDA Enzyme Link: BRENDA 2.8.4.5
KEGG Enzyme Link: KEGG2.8.4.5
BioCyc Enzyme Link: BioCyc 2.8.4.5
ExPASy Enzyme Link: ExPASy2.8.4.5
EC2PDB Enzyme Link: EC2PDB 2.8.4.5
ExplorEnz Enzyme Link: ExplorEnz 2.8.4.5
PRIAM enzyme-specific profiles Link: PRIAM 2.8.4.5
IntEnz Enzyme Link: IntEnz 2.8.4.5
MEDLINE Enzyme Link: MEDLINE 2.8.4.5

EC number:2.8.4.5

MSA:

2.8.4.5;
Phylogenetic Tree:

2.8.4.5;
Uniprot:
M-CSA:
RHEA:37075 [sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine
RULE(radius=1) [*;H0;+0:1]-[SH;+0:2].[*:3]-[S+;H0:4](-[*:5])-[CH2;+0:6]-[*:7].[*:8]-[S+;H0:9](-[*:10])-[CH3;+0:11].[*:12]:[cH;+0:13]:[*:14]>>[*;H0;+0:1].[*:7]-[CH3;+0:6].[*:8]-[S;H0;+0:9]-[*:10].[*:3]-[S;H0;+0:4]-[*:5].[*:12]:[c;H0;+0:13](:[*:14])-[S;H0;+0:2]-[CH3;+0:11]
Reaction
Core-to-Core No scaffolds atoms were exchanged as a result of the reaction

References

TitleAuthorsDatePubMed ID
Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP.Lauhon CT2012 Nov 623072323
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.Arragain S, Handelman SK, Forouhar F, Wei FY, Tomizawa K, Hunt JF, Douki T, Fontecave M, Mulliez E, Atta M2010 Sep 1020584901