EC number:3.2.1.169
Enzyme
Download| EC Tree |
| 3. Hydrolases |
| 3.2 Glycosylases |
| 3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds |
| ID: | 3.2.1.169 |
|---|---|
| Description: | Protein O-GlcNAcase. |
| Alternative Name: |
OGA. O-GlcNAcase. O-GlcNAc hydrolase. Glycoside hydrolase O-GlcNAcase. |
| Cath: | 3.20.20.80; 3.30.379.10; 3.30.720.150; 1.20.58.240; 1.20.58.460; 1.25.40.10; 3.40.50.11380; 3.40.50.20; 3.40.50.200; 3.40.50.2000; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 3.2.1.169 |
| BRENDA Enzyme Link: | BRENDA 3.2.1.169 |
| KEGG Enzyme Link: | KEGG3.2.1.169 |
| BioCyc Enzyme Link: | BioCyc 3.2.1.169 |
| ExPASy Enzyme Link: | ExPASy3.2.1.169 |
| EC2PDB Enzyme Link: | EC2PDB 3.2.1.169 |
| ExplorEnz Enzyme Link: | ExplorEnz 3.2.1.169 |
| PRIAM enzyme-specific profiles Link: | PRIAM 3.2.1.169 |
| IntEnz Enzyme Link: | IntEnz 3.2.1.169 |
| MEDLINE Enzyme Link: | MEDLINE 3.2.1.169 |
EC number:3.2.1.169
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:48892 | 3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H2O = L-threonyl-[protein] + N-acetyl-D-glucosamine |
| RULE(radius=1) | [*:1]-[CH;+0:2](-[*:3])-[O;H0;+0:4]-[*:5].[OH2;+0:6]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:6].[*:5]-[OH;+0:4] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol. | Dong DL, Hart GW | 1994 Jul 29 | 8034696 |
| Synergy of peptide and sugar in O-GlcNAcase substrate recognition. | Schimpl M, Borodkin VS, Gray LJ, van Aalten DM | 2012 Feb 24 | 22365600 |
| Human OGA binds substrates in a conserved peptide recognition groove. | Schimpl M, Schüttelkopf AW, Borodkin VS, van Aalten DM | 2010 Nov 15 | 20863279 |
| Isoforms of human O-GlcNAcase show distinct catalytic efficiencies. | Li J, Huang CL, Zhang LW, Lin L, Li ZH, Zhang FW, Wang P | 2010 Jul | 20673219 |
| Chemical dissection of the link between streptozotocin, O-GlcNAc, and pancreatic cell death. | Pathak S, Dorfmueller HC, Borodkin VS, van Aalten DM | 2008 Aug 25 | 18721751 |
| Characterization of beta-N-acetylglucosaminidase cleavage by caspase-3 during apoptosis. | Butkinaree C, Cheung WD, Park S, Park K, Barber M, Hart GW | 2008 Aug 29 | 18586680 |
| Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate. | Kim EJ, Kang DO, Love DC, Hanover JA | 2006 Jun 12 | 16584714 |
| Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity. | Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GN, Vocadlo DJ, Davies GJ | 2006 Apr | 16565725 |
| Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis. | Rao FV, Dorfmueller HC, Villa F, Allwood M, Eggleston IM, van Aalten DM | 2006 Apr 5 | 16541109 |
| Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants. | Cetinbaş N, Macauley MS, Stubbs KA, Drapala R, Vocadlo DJ | 2006 Mar 21 | 16533067 |
| Location and characterization of the O-GlcNAcase active site. | Toleman C, Paterson AJ, Kudlow JE | 2006 May | 16517082 |
| Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase. | Wells L, Gao Y, Mahoney JA, Vosseller K, Chen C, Rosen A, Hart GW | 2002 Jan 18 | 11788610 |
| Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain. | Gao Y, Wells L, Comer FI, Parker GJ, Hart GW | 2001 Mar 30 | 11148210 |
| RHEA:48876 | 3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H2O = L-seryl-[protein] + N-acetyl-D-glucosamine |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[O;H0;+0:3]-[*:4].[OH2;+0:5]>>[*:1]-[CH2;+0:2]-[OH;+0:5].[*:4]-[OH;+0:3] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol. | Dong DL, Hart GW | 1994 Jul 29 | 8034696 |
| Synergy of peptide and sugar in O-GlcNAcase substrate recognition. | Schimpl M, Borodkin VS, Gray LJ, van Aalten DM | 2012 Feb 24 | 22365600 |
| Human OGA binds substrates in a conserved peptide recognition groove. | Schimpl M, Schüttelkopf AW, Borodkin VS, van Aalten DM | 2010 Nov 15 | 20863279 |
| Isoforms of human O-GlcNAcase show distinct catalytic efficiencies. | Li J, Huang CL, Zhang LW, Lin L, Li ZH, Zhang FW, Wang P | 2010 Jul | 20673219 |
| Chemical dissection of the link between streptozotocin, O-GlcNAc, and pancreatic cell death. | Pathak S, Dorfmueller HC, Borodkin VS, van Aalten DM | 2008 Aug 25 | 18721751 |
| Characterization of beta-N-acetylglucosaminidase cleavage by caspase-3 during apoptosis. | Butkinaree C, Cheung WD, Park S, Park K, Barber M, Hart GW | 2008 Aug 29 | 18586680 |
| Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate. | Kim EJ, Kang DO, Love DC, Hanover JA | 2006 Jun 12 | 16584714 |
| Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity. | Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GN, Vocadlo DJ, Davies GJ | 2006 Apr | 16565725 |
| Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis. | Rao FV, Dorfmueller HC, Villa F, Allwood M, Eggleston IM, van Aalten DM | 2006 Apr 5 | 16541109 |
| Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants. | Cetinbaş N, Macauley MS, Stubbs KA, Drapala R, Vocadlo DJ | 2006 Mar 21 | 16533067 |
| Location and characterization of the O-GlcNAcase active site. | Toleman C, Paterson AJ, Kudlow JE | 2006 May | 16517082 |
| Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase. | Wells L, Gao Y, Mahoney JA, Vosseller K, Chen C, Rosen A, Hart GW | 2002 Jan 18 | 11788610 |
| Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain. | Gao Y, Wells L, Comer FI, Parker GJ, Hart GW | 2001 Mar 30 | 11148210 |