EC number:4.1.2.47
| ID: | 4.1.2.47 |
|---|---|
| Description: | (S)-hydroxynitrile lyase. |
| Alternative Name: |
Oxynitrilase. Hydroxynitrile lyase. (S)-oxynitrilase. (S)-cyanohydrin producing hydroxynitrile lyase. |
| Cath: | 1.20.5.2390; 3.40.50.11320; 3.40.50.1820; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 4.1.2.47 |
| BRENDA Enzyme Link: | BRENDA 4.1.2.47 |
| KEGG Enzyme Link: | KEGG4.1.2.47 |
| BioCyc Enzyme Link: | BioCyc 4.1.2.47 |
| ExPASy Enzyme Link: | ExPASy4.1.2.47 |
| EC2PDB Enzyme Link: | EC2PDB 4.1.2.47 |
| ExplorEnz Enzyme Link: | ExplorEnz 4.1.2.47 |
| PRIAM enzyme-specific profiles Link: | PRIAM 4.1.2.47 |
| IntEnz Enzyme Link: | IntEnz 4.1.2.47 |
| MEDLINE Enzyme Link: | MEDLINE 4.1.2.47 |
| RHEA:56592 | a disubstituted aliphatic (S)-hydroxynitrile = a ketone + hydrogen cyanide |
| RULE(radius=1) | [*:1]-[C;H0;+0:2](-[*:3])(-[OH;+0:4])-[C;H0;+0:5]#[*:6]>>[*:6]#[CH;+0:5].[*:1]-[C;H0;+0:2](-[*:3])=[O;H0;+0:4] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4'-oxocyclohexyl)acetonitrile as the model. | Avi M, Wiedner RM, Griengl H, Schwab H | 2008 | 19006143 |
| Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor. | Semba H, Dobashi Y, Matsui T | 2008 Jun | 18540112 |
| Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis. | Schmidt A, Gruber K, Kratky C, Lamzin VS | 2008 Aug 1 | 18524775 |
| Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values. | von Langermann J, Guterl JK, Pohl M, Wajant H, Kragl U | 2008 Apr | 18204865 |
| Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis. | Gartler G, Kratky C, Gruber K | 2007 Mar 30 | 17250917 |
| Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis. | Wagner UG, Schall M, Hasslacher M, Hayn M, Griengl H, Schwab H, Kratky C | 1996 May 1 | 15299689 |
| Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta. | Bühler H, Effenberger F, Förster S, Roos J, Wajant H | 2003 Mar 3 | 12616635 |
| RHEA:56588 | a monosubstituted aliphatic (S)-hydroxynitrile = an aldehyde + hydrogen cyanide |
| RULE(radius=1) | [*:1]-[CH;+0:2](-[OH;+0:3])-[C;H0;+0:4]#[*:5]>>[*:5]#[CH;+0:4].[*:1]-[CH;+0:2]=[O;H0;+0:3] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4'-oxocyclohexyl)acetonitrile as the model. | Avi M, Wiedner RM, Griengl H, Schwab H | 2008 | 19006143 |
| Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor. | Semba H, Dobashi Y, Matsui T | 2008 Jun | 18540112 |
| Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis. | Schmidt A, Gruber K, Kratky C, Lamzin VS | 2008 Aug 1 | 18524775 |
| Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values. | von Langermann J, Guterl JK, Pohl M, Wajant H, Kragl U | 2008 Apr | 18204865 |
| Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis. | Gartler G, Kratky C, Gruber K | 2007 Mar 30 | 17250917 |
| Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis. | Wagner UG, Schall M, Hasslacher M, Hayn M, Griengl H, Schwab H, Kratky C | 1996 May 1 | 15299689 |
| Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta. | Bühler H, Effenberger F, Förster S, Roos J, Wajant H | 2003 Mar 3 | 12616635 |
| RHEA:54660 | an aromatic (S)-hydroxynitrile = an aromatic aldehyde + hydrogen cyanide |
| RULE(radius=1) | [*:1]-[CH;+0:2](-[OH;+0:3])-[C;H0;+0:4]#[*:5]>>[*:5]#[CH;+0:4].[*:1]-[CH;+0:2]=[O;H0;+0:3] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4'-oxocyclohexyl)acetonitrile as the model. | Avi M, Wiedner RM, Griengl H, Schwab H | 2008 | 19006143 |
| Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor. | Semba H, Dobashi Y, Matsui T | 2008 Jun | 18540112 |
| Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis. | Schmidt A, Gruber K, Kratky C, Lamzin VS | 2008 Aug 1 | 18524775 |
| Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values. | von Langermann J, Guterl JK, Pohl M, Wajant H, Kragl U | 2008 Apr | 18204865 |
| Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis. | Gartler G, Kratky C, Gruber K | 2007 Mar 30 | 17250917 |
| Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis. | Wagner UG, Schall M, Hasslacher M, Hayn M, Griengl H, Schwab H, Kratky C | 1996 May 1 | 15299689 |
| Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta. | Bühler H, Effenberger F, Förster S, Roos J, Wajant H | 2003 Mar 3 | 12616635 |