Enzyme

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     4. Lyases
        4.1 Carbon-carbon lyases
            4.1.3 Oxo-acid-lyases
ID:4.1.3.4
Description:Hydroxymethylglutaryl-CoA lyase.
Alternative Name: HMG-CoA lyase.
3-hydroxy-3-methylglutarate-CoA lyase.
(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetate-lyase.
Prosite: PDOC00813;
PDB:
PDBScop
3MP5
3MP4
3MP3
2FTP
2CW6
Cath: 3.20.20.70;

3D structure

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References

External Links

UniProtKB Enzyme Link: UniProtKB 4.1.3.4
BRENDA Enzyme Link: BRENDA 4.1.3.4
KEGG Enzyme Link: KEGG4.1.3.4
BioCyc Enzyme Link: BioCyc 4.1.3.4
ExPASy Enzyme Link: ExPASy4.1.3.4
EC2PDB Enzyme Link: EC2PDB 4.1.3.4
ExplorEnz Enzyme Link: ExplorEnz 4.1.3.4
PRIAM enzyme-specific profiles Link: PRIAM 4.1.3.4
IntEnz Enzyme Link: IntEnz 4.1.3.4
MEDLINE Enzyme Link: MEDLINE 4.1.3.4
MSA:

4.1.3.4;

Phylogenetic Tree:

4.1.3.4;

Uniprot:
M-CSA:
RHEA:24404 (3S)-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA
RULE(radius=1) [*:1]-[C;H0;+0:2](-[*:3])(-[OH;+0:4])-[CH2;+0:5]-[*:6]>>[*:1]-[C;H0;+0:2](-[*:3])=[O;H0;+0:4].[*:6]-[CH3;+0:5]
Reaction
Core-to-Core No scaffolds atoms were exchanged as a result of the reaction

References

TitleAuthorsDatePubMed ID
3-Hydroxy-3-methylglutaryl-CoA lyase: expression and isolation of the recombinant human enzyme and investigation of a mechanism for regulation of enzyme activity.Roberts JR, Narasimhan C, Hruz PW, Mitchell GA, Miziorko HM1994 Jul 88027038
Identification and characterization of an extramitochondrial human 3-hydroxy-3-methylglutaryl-CoA lyase.Montgomery C, Pei Z, Watkins PA, Miziorko HM2012 Sep 2822865860
Characterization of a novel HMG-CoA lyase enzyme with a dual location in endoplasmic reticulum and cytosol.Arnedo M, Menao S, Puisac B, Teresa-Rodrigo ME, Gil-Rodríguez MC, López-Viñas E, Gómez-Puertas P, Casals N, Casale CH, Hegardt FG, Pié J2012 Oct22847177
3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL). Cloning of human and chicken liver HL cDNAs and characterization of a mutation causing human HL deficiency.Mitchell GA, Robert MF, Hruz PW, Wang S, Fontaine G, Behnke CE, Mende-Mueller LM, Schappert K, Lee C, Gibson KM, Miziorko HM1993 Feb 258440722
The bifunctional role of LiuE from Pseudomonas aeruginosa, displays additionally HIHG-CoA lyase enzymatic activity.Chávez-Avilés M, Díaz-Pérez AL, Campos-García J2010 Apr19597963
Evaluation of 3-hydroxy-3-methylglutaryl-coenzyme A lyase arginine-41 as a catalytic residue: use of acetyldithio-coenzyme A to monitor product enolization.Tuinstra RL, Wang CZ, Mitchell GA, Miziorko HM2004 May 1115122894
Avian 3-hydroxy-3-methylglutaryl-CoA lyase: sensitivity of enzyme activity to thiol/disulfide exchange and identification of proximal reactive cysteines.Hruz PW, Miziorko HM1992 Sep1304393