Enzyme

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     4. Lyases
        4.2 Carbon-oxygen lyases
            4.2.3 Acting on phosphates
ID:4.2.3.124
Description:2-deoxy-scyllo-inosose synthase.
Cath: 1.20.1090.10; 3.40.50.1970;

3D structure

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References

External Links

UniProtKB Enzyme Link: UniProtKB 4.2.3.124
BRENDA Enzyme Link: BRENDA 4.2.3.124
KEGG Enzyme Link: KEGG4.2.3.124
BioCyc Enzyme Link: BioCyc 4.2.3.124
ExPASy Enzyme Link: ExPASy4.2.3.124
EC2PDB Enzyme Link: EC2PDB 4.2.3.124
ExplorEnz Enzyme Link: ExplorEnz 4.2.3.124
PRIAM enzyme-specific profiles Link: PRIAM 4.2.3.124
IntEnz Enzyme Link: IntEnz 4.2.3.124
MEDLINE Enzyme Link: MEDLINE 4.2.3.124
MSA:

4.2.3.124;

Phylogenetic Tree:

4.2.3.124;

Uniprot:
M-CSA:
RHEA:33071 D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
RULE(radius=1) [*:1]-[CH;+0:2](-[*:3])-[O;H0;+0:4]-[CH;+0:5](-[*:6])-[CH2;+0:7]-[O;H0;+0:8]-[*:9]>>[*:1]-[CH;+0:2](-[*:3])-[C;H0;+0:7](=[O;H0;+0:4])-[CH2;+0:5]-[*:6].[*:9]-[OH;+0:8]
Reaction
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans.Kudo F, Yamauchi N, Suzuki R, Kakinuma K1997 May9207913
Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism.Tamegai H, Sawada H, Nango E, Aoki R, Hirakawa H, Iino T, Eguchi T201020530911
Structure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and NAD+.Nango E, Kumasaka T, Hirayama T, Tanaka N, Eguchi T2008 Feb 117879343
Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans.Thuy ML, Kharel MK, Lamichhane R, Lee HC, Suh JW, Liou K, Sohng JK2005 Apr15928851
Stereospecificity of hydride transfer in NAD+-catalyzed 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics.Huang Z, Kakinuma K, Eguchi T2005 Apr15788164
Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans.Tamegai H, Nango E, Koike-Takeshita A, Kudo F, Kakinuma K2002 Jul12224638
Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase.Kudo F, Tamegai H, Fujiwara T, Tagami U, Hirayama K, Kakinuma K1999 Jun10470681
Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics.Kudo F, Hosomi Y, Tamegai H, Kakinuma K1999 Feb10344560