EC number:6.3.2.17
Enzyme
Download| EC Tree |
| 6. Ligases |
| 6.3 Forming carbon-nitrogen bonds |
| 6.3.2 Acid-D-amino-acid ligases (peptide synthases) |
| ID: | 6.3.2.17 |
|---|---|
| Description: | Tetrahydrofolate synthase. |
| Alternative Name: |
Tetrahydrofolylpolyglutamate synthase. Tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming). N(10)-formyltetrahydropteroyldiglutamate synthetase. FPGS. Formyltetrahydropteroyldiglutamate synthetase. Folylpolyglutamyl synthetase. Folylpolyglutamate synthetase. Folylpolyglutamate synthase. Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Folylpoly-gamma-glutamate synthase. Folylpoly(gamma-glutamate) synthase. Folate polyglutamate synthetase. |
| Prosite: | PDOC00773; |
| PDB: |
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| Cath: | 3.40.1190.10; 3.90.190.20; |
3D structure
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Note: Use your mouse to drag, rotate, and zoom in and out of the structure. Mouse-over to identify atoms and bonds. Mouse controls documentation.References
External Links
| UniProtKB Enzyme Link: | UniProtKB 6.3.2.17 |
| BRENDA Enzyme Link: | BRENDA 6.3.2.17 |
| KEGG Enzyme Link: | KEGG6.3.2.17 |
| BioCyc Enzyme Link: | BioCyc 6.3.2.17 |
| ExPASy Enzyme Link: | ExPASy6.3.2.17 |
| EC2PDB Enzyme Link: | EC2PDB 6.3.2.17 |
| ExplorEnz Enzyme Link: | ExplorEnz 6.3.2.17 |
| PRIAM enzyme-specific profiles Link: | PRIAM 6.3.2.17 |
| IntEnz Enzyme Link: | IntEnz 6.3.2.17 |
| MEDLINE Enzyme Link: | MEDLINE 6.3.2.17 |
| RHEA:51912 | (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate |
| RULE(radius=1) | [*:1]-[NH2;+0:2].[*:3]=[C;H0;+0:4](-[*:5])-[OH;+0:6].[*:7]=[P;H0;+0:8](-[*:9])(-[*:10])-[O;H0;+0:11]-[*:12]>>[*:12]-[OH;+0:11].[*:3]=[C;H0;+0:4](-[*:5])-[NH;+0:2]-[*:1].[*:7]=[P;H0;+0:8](-[*:9])(-[*:10])-[OH;+0:6] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and properties of Lactobacillus casei folylpoly-gamma-glutamate synthetase. | Bognar AL, Shane B | 1983 Oct 25 | 6138353 |
| Mutagenesis of the folC gene encoding folylpolyglutamate synthetase-dihydrofolate synthetase in Escherichia coli. | Kimlova LJ, Pyne C, Keshavjee K, Huy J, Beebakhee G, Bognar AL | 1991 Jan | 1989505 |
| Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product. | Bognar AL, Osborne C, Shane B, Singer SC, Ferone R | 1985 May 10 | 2985605 |
| RHEA:51904 | 10-formyltetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = 10-formyltetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate |
| RULE(radius=1) | [*:1]-[C;H0;+0:2](=[*:3])-[OH;+0:4].[*:5]-[NH2;+0:6].[*:7]=[P;H0;+0:8](-[*:9])(-[*:10])-[O;H0;+0:11]-[*:12]>>[*:1]-[C;H0;+0:2](=[*:3])-[NH;+0:6]-[*:5].[*:12]-[OH;+0:11].[*:7]=[P;H0;+0:8](-[*:9])(-[*:10])-[OH;+0:4] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and properties of Lactobacillus casei folylpoly-gamma-glutamate synthetase. | Bognar AL, Shane B | 1983 Oct 25 | 6138353 |
| Mutagenesis of the folC gene encoding folylpolyglutamate synthetase-dihydrofolate synthetase in Escherichia coli. | Kimlova LJ, Pyne C, Keshavjee K, Huy J, Beebakhee G, Bognar AL | 1991 Jan | 1989505 |
| Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product. | Bognar AL, Osborne C, Shane B, Singer SC, Ferone R | 1985 May 10 | 2985605 |
| RHEA:10580 | (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)n + ATP + L-glutamate = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)n+1 + ADP + H(+) + phosphate |
| RULE(radius=1) | [*:1]-[NH2;+0:2].[*:3]=[C;H0;+0:4](-[*:5])-[OH;+0:6].[*:7]=[P;H0;+0:8](-[*:9])(-[*:10])-[O;H0;+0:11]-[*:12]>>[*:12]-[OH;+0:11].[*:3]=[C;H0;+0:4](-[*:5])-[NH;+0:2]-[*:1].[*:7]=[P;H0;+0:8](-[*:9])(-[*:10])-[OH;+0:6] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene. | Chen L, Qi H, Korenberg J, Garrow TA, Choi YJ, Shane B | 1996 May 31 | 8662720 |
| Regulation of folate and one-carbon metabolism in mammalian cells. III. Role of mitochondrial folylpoly-gamma-glutamate synthetase. | Lin BF, Huang RF, Shane B | 1993 Oct 15 | 8408020 |
| Regulation of folate and one-carbon metabolism in mammalian cells. I. Folate metabolism in Chinese hamster ovary cells expressing Escherichia coli or human folylpoly-gamma-glutamate synthetase activity. | Osborne CB, Lowe KE, Shane B | 1993 Oct 15 | 8408018 |
| Different antifolate-resistant L1210 cell variants with either increased or decreased folylpolyglutamate synthetase gene expression at the level of mRNA transcription. | Roy K, Mitsugi K, Sirlin S, Shane B, Sirotnak FM | 1995 Nov 10 | 7592937 |
| Concentration-dependent processivity of multiple glutamate ligations catalyzed by folylpoly-gamma-glutamate synthetase. | Tomsho JW, Moran RG, Coward JK | 2008 Aug 26 | 18672898 |
| Mutagenesis of folylpolyglutamate synthetase indicates that dihydropteroate and tetrahydrofolate bind to the same site. | Sheng Y, Khanam N, Tsaksis Y, Shi XM, Lu QS, Bognar AL | 2008 Feb 26 | 18232714 |
| Identification and characterization of genetic variation in the folylpolyglutamate synthase gene. | Leil TA, Endo C, Adjei AA, Dy GK, Salavaggione OE, Reid JR, Ames MM, Adjei AA | 2007 Sep 15 | 17875718 |
| Expression cloning of a human cDNA encoding folylpoly(gamma-glutamate) synthetase and determination of its primary structure. | Garrow TA, Admon A, Shane B | 1992 Oct 1 | 1409616 |