EC number:6.3.5.6
Enzyme
Download| EC Tree |
| 6. Ligases |
| 6.3 Forming carbon-nitrogen bonds |
| 6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor |
| ID: | 6.3.5.6 |
|---|---|
| Description: | Asparaginyl-tRNA synthase (glutamine-hydrolyzing). |
| Alternative Name: |
GatCAB. Aspartyl-tRNA(Asn) amidotransferase. Asp-tRNA(Asn) amidotransferase. Asp-AdT. |
| Cath: | 3.30.1360.30; 3.30.930.10; 2.40.50.140; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 6.3.5.6 |
| BRENDA Enzyme Link: | BRENDA 6.3.5.6 |
| KEGG Enzyme Link: | KEGG6.3.5.6 |
| BioCyc Enzyme Link: | BioCyc 6.3.5.6 |
| ExPASy Enzyme Link: | ExPASy6.3.5.6 |
| EC2PDB Enzyme Link: | EC2PDB 6.3.5.6 |
| ExplorEnz Enzyme Link: | ExplorEnz 6.3.5.6 |
| PRIAM enzyme-specific profiles Link: | PRIAM 6.3.5.6 |
| IntEnz Enzyme Link: | IntEnz 6.3.5.6 |
| MEDLINE Enzyme Link: | MEDLINE 6.3.5.6 |
| RHEA:14513 | ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate |
| RULE(radius=1) | [*:1]-[C;H0;+0:2](=[*:3])-[NH2;+0:4].[*:5]-[C;H0;+0:6](=[*:7])-[OH;+0:8].[*:9]=[P;H0;+0:10](-[*:11])(-[*:12])-[O;H0;+0:13]-[*:14].[OH2;+0:15]>>[*:5]-[C;H0;+0:6](=[*:7])-[NH2;+0:4].[*:1]-[C;H0;+0:2](=[*:3])-[OH;+0:8].[*:14]-[OH;+0:13].[*:9]=[P;H0;+0:10](-[*:11])(-[*:12])-[OH;+0:15] |
| Reaction |  |
| Core-to-Core | No scaffolds atoms were exchanged as a result of the reaction |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Glutamyl-tRNA(Gln) amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis. | Curnow AW, Tumbula DL, Pelaschier JT, Min B, Söll D | 1998 Oct 27 | 9789001 |
| Aminoacyl-tRNA synthesis. | Ibba M, Soll D | 2000 | 10966471 |
| Insights into tRNA-dependent amidotransferase evolution and catalysis from the structure of the Aquifex aeolicus enzyme. | Wu J, Bu W, Sheppard K, Kitabatake M, Kwon ST, Söll D, Smith JL | 2009 Aug 28 | 19520089 |
| Ammonia channel couples glutaminase with transamidase reactions in GatCAB. | Nakamura A, Yao M, Chimnaronk S, Sakai N, Tanaka I | 2006 Jun 30 | 16809541 |
| Transfer RNA-dependent amino acid biosynthesis: an essential route to asparagine formation. | Min B, Pelaschier JT, Graham DE, Tumbula-Hansen D, Söll D | 2002 Mar 5 | 11880622 |
| A single amidotransferase forms asparaginyl-tRNA and glutaminyl-tRNA in Chlamydia trachomatis. | Raczniak G, Becker HD, Min B, Söll D | 2001 Dec 7 | 11585842 |