EC number:1.14.14.32
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.14.32 |
|---|---|
| Description: | 17-alpha-hydroxyprogesterone deacetylase. |
| Alternative Name: |
CYP17A1. CYP17. 17-alpha-hydroxyprogesterone acetaldehyde-lyase. 17-alpha-hydroxyprogesterone 17,20-lyase. |
| Cath: | 1.10.630.10; |
3D structure
Click one PDB to see exact 3D structure provided by NGL.
Note: Use your mouse to drag, rotate, and zoom in and out of the structure. Mouse-over to identify atoms and bonds. Mouse controls documentation.References
External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.14.32 |
| BRENDA Enzyme Link: | BRENDA 1.14.14.32 |
| KEGG Enzyme Link: | KEGG1.14.14.32 |
| BioCyc Enzyme Link: | BioCyc 1.14.14.32 |
| ExPASy Enzyme Link: | ExPASy1.14.14.32 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.14.32 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.14.32 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.14.32 |
| IntEnz Enzyme Link: | IntEnz 1.14.14.32 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.14.32 |
EC number:1.14.14.32
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:50244 | 17alpha-hydroxypregnenolone + O2 + reduced [NADPH--hemoprotein reductase] = 3beta-hydroxyandrost-5-en-17-one + acetate + 2 H(+) + H2O + oxidized [NADPH--hemoprotein reductase] |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[*:3]:[*:4]-[NH;+0:5]-[c;H0;+0:6](:[*:7]):[c;H0;+0:8]-1:[nH;+0:9]:[*:10].[*:11]=[C;H0;+0:12](-[*:13])-[C;H0;+0:14](-[*:15])(-[*:16])-[OH;+0:17].[O;H0;+0:18]=[O;H0;+0:19]>>[*:15]-[C;H0;+0:14](-[*:16])=[O;H0;+0:17].[*:1]-[n;H0;+0:2]1:[*:3]:[*:4]:[n;H0;+0:5]:[c;H0;+0:6](:[*:7])-[c;H0;+0:8]:1:[n;H0;+0:9]:[*:10].[*:11]=[C;H0;+0:12](-[*:13])-[OH;+0:18].[OH2;+0:19] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Cytochrome b5 augments the 17,20-lyase activity of human P450c17 without direct electron transfer. | Auchus RJ, Lee TC, Miller WL | 1998 Feb 6 | 9452426 |
| Mechanism of 17α,20-Lyase and New Hydroxylation Reactions of Human Cytochrome P450 17A1: 18O LABELING AND OXYGEN SURROGATE EVIDENCE FOR A ROLE OF A PERFERRYL OXYGEN. | Yoshimoto FK, Gonzalez E, Auchus RJ, Guengerich FP | 2016 Aug 12 | 27339894 |
| Role of cytochrome b5 in the modulation of the enzymatic activities of cytochrome P450 17α-hydroxylase/17,20-lyase (P450 17A1). | Bhatt MR, Khatri Y, Rodgers RJ, Martin LL | 2017 Jun | 26976652 |
| Unveiling the crucial intermediates in androgen production. | Mak PJ, Gregory MC, Denisov IG, Sligar SG, Kincaid JR | 2015 Dec 29 | 26668369 |
| RHEA:14753 | 17alpha-hydroxyprogesterone + O2 + reduced [NADPH--hemoprotein reductase] = acetate + androst-4-ene-3,17-dione + 2 H(+) + H2O + oxidized [NADPH--hemoprotein reductase] |
| RULE(radius=1) | [*:1]-[C;H0;+0:2](-[*:3])(-[OH;+0:4])-[C;H0;+0:5](=[*:6])-[*:7]>>[*:1]-[C;H0;+0:2](-[*:3])=[O;H0;+0:4].[*:6]=[CH;+0:5]-[*:7] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Cytochrome b5 augments the 17,20-lyase activity of human P450c17 without direct electron transfer. | Auchus RJ, Lee TC, Miller WL | 1998 Feb 6 | 9452426 |
| Bioconversion of steroids in vitro by testes from autoimmunized rabbits. | Nowotny E, Sananez RD, Nattero G, Yantorno C, Faillaci MG | 1974 Jun | 4435747 |
| Mechanism of 17α,20-Lyase and New Hydroxylation Reactions of Human Cytochrome P450 17A1: 18O LABELING AND OXYGEN SURROGATE EVIDENCE FOR A ROLE OF A PERFERRYL OXYGEN. | Yoshimoto FK, Gonzalez E, Auchus RJ, Guengerich FP | 2016 Aug 12 | 27339894 |
| Role of cytochrome b5 in the modulation of the enzymatic activities of cytochrome P450 17α-hydroxylase/17,20-lyase (P450 17A1). | Bhatt MR, Khatri Y, Rodgers RJ, Martin LL | 2017 Jun | 26976652 |
| Unveiling the crucial intermediates in androgen production. | Mak PJ, Gregory MC, Denisov IG, Sligar SG, Kincaid JR | 2015 Dec 29 | 26668369 |
| Mechanistic Scrutiny Identifies a Kinetic Role for Cytochrome b5 Regulation of Human Cytochrome P450c17 (CYP17A1, P450 17A1). | Simonov AN, Holien JK, Yeung JC, Nguyen AD, Corbin CJ, Zheng J, Kuznetsov VL, Auchus RJ, Conley AJ, Bond AM, Parker MW, Rodgers RJ, Martin LL | 2015 | 26587646 |
| Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001. | DeVore NM, Scott EE | 2012 Jan 22 | 22266943 |
| Engineering, expression, and purification of "soluble" human cytochrome P45017alpha and its functional characterization. | Pechurskaya TA, Lukashevich OP, Gilep AA, Usanov SA | 2008 Jul | 18707589 |
| Functional expression and characterisation of human cytochrome P45017alpha in Pichia pastoris. | Kolar NW, Swart AC, Mason JI, Swart P | 2007 May 10 | 17386955 |
| Molecular cloning and heterologous expression in E. coli of cytochrome P45017alpha. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species. | Gilep AA, Estabrook RW, Usanov SA | 2003 Jan | 12693981 |