EC number:1.14.20.1
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated |
| ID: | 1.14.20.1 | ||
|---|---|---|---|
| Description: | Deacetoxycephalosporin-C synthase. | ||
| Alternative Name: |
Penicillin N expandase. DAOCS. DAOC synthase. | ||
| Prosite: | PDOC51471; PDOC00165; | ||
| PDB: |
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| Cath: | 2.60.120.330; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.20.1 |
| BRENDA Enzyme Link: | BRENDA 1.14.20.1 |
| KEGG Enzyme Link: | KEGG1.14.20.1 |
| BioCyc Enzyme Link: | BioCyc 1.14.20.1 |
| ExPASy Enzyme Link: | ExPASy1.14.20.1 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.20.1 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.20.1 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.20.1 |
| IntEnz Enzyme Link: | IntEnz 1.14.20.1 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.20.1 |
EC number:1.14.20.1
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:20748 | 2-oxoglutarate + O2 + penicillin N = CO2 + deacetoxycephalosporin C + H2O + succinate |
| RULE(radius=1) | [*:1]-[C;H0;+0:2]1(-[CH3;+0:3])-[S;H0;+0:4]-[*:5]-[*:6]-[CH;+0:7]-1-[*:8].[*:9]=[C;H0;+0:10](-[OH;+0:11])-[C;H0;+0:12](=[*:13])-[*:14].[O;H0;+0:15]=[O;H0;+0:16]>>[*:1]-[C;H0;+0:2]1=[C;H0;+0:7](-[*:8])-[*:6]-[*:5]-[S;H0;+0:4]-[CH2;+0:3]-1.[*:13]=[C;H0;+0:12](-[*:14])-[OH;+0:15].[*:9]=[C;H0;+0:10]=[O;H0;+0:11].[OH2;+0:16] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus. | Dotzlaf JE, Yeh WK | 1989 Jun 15 | 2656705 |
| Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: construction of a new fungal biosynthetic pathway. | Cantwell C, Beckmann R, Whiteman P, Queener SW, Abraham EP | 1992 Jun 22 | 1354366 |
| Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS). | Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ | 2001 May 18 | 11352583 |